Coding
RUNX2

Part:BBa_K415501:Design

Designed by: Laura Deming, Joy Jiao, Adrian Slusarczyk   Group: iGEM10_MIT   (2010-10-24)


RUNX2 L1L2 MammoBlock Entry Vector


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1533
    Illegal PstI site found at 112
    Illegal PstI site found at 275
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1533
    Illegal PstI site found at 112
    Illegal PstI site found at 275
    Illegal NotI site found at 291
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1533
    Illegal BamHI site found at 1366
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1533
    Illegal PstI site found at 112
    Illegal PstI site found at 275
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1533
    Illegal PstI site found at 112
    Illegal PstI site found at 275
    Illegal NgoMIV site found at 83
    Illegal AgeI site found at 1400
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1403
    Illegal BsaI.rc site found at 1397
    Illegal SapI site found at 1295
    Illegal SapI.rc site found at 1086


Design Notes

We wanted to use the longest RUNX2 isoform; this isoform has been shown to induce bone differentiation when transfected into human cells.


Source

Obtained as an L1L2 Entry vector from Open Biosystems.

References